基于UFLC-Q-TOF-MS/MS技术分析障眼明片浸膏的化学成分和入血成分OA

To comprehensively investigate the chemical components and constituents in plasma of Zhangyanming Tablet extract using ultra-fast liquid chromatography-quadrupole-time-of-flight tandem mass spectrometry(UFLC-Q-TOF-MS/MS).Gradient elution was performed using mobile phase consisting of water and acetonitrile,both containing φ=0.1％formic acid.Separation of chemical components and constituents in plasma was conducted on Hypersil GOLD(4.6 mm×250 mm,5 μm)and Kinetex C18(2.1 mm×100 mm,2.6 μm)column,with flow rate of 1.0 and 0.3 mL/min,and column temperature maintained at 35 and 40 ℃,respectively.Electrospray ionization(ESI)source was used in both positive and negative ion mode,with a scanning m/z range from 50 to 1 500.Through analyzing retention time,adduct ion peak,MS/MS fragments,and comparison with reference standards,database,and literature data,a total of 200 chemical compounds were identified in Zhangyanming Tablet extract,including 47 flavonoids,41 terpenoids,31 organic acids,20 phenylpropanoids,17 alkaloids,10 saccharides,8 amino acids,4 anthraquinones,4 phthalides,3 coumarins and 15 other components.In Beagle dog plasma,a total of 46 chemical constituents were detected,among which 39 were prototype compounds and 7 were metabolites.This study established a systematic and efficient approach for the separation and identification of chemical components and constituents in plasma of Zhangyanming Tablet extract.The study summarized the characteristic fragmentation patterns of major compound types,providing scientific evidence and references for investigating the pharmacologically active substances and quality evaluation of Zhangyanming Tablet extract.