不同光照条件对纤花香茶菜生长发育和次生代谢物质的影响OA

To study the effects of different shading treatments on the agronomic traits and chemical component contents and explore the suitable light conditions for the yield and quality of the Isodon lophanthoides var.graciliflorus(Benth.)H.Hara,and provide a scientific basis for the standardized planting techniques of Ⅰ.lophanthoides var.graciliflorus.Taking the seedlings of the seeds of Ⅰ.lophanthoides var.graciliflorus as materials,4 treatments of 100％(CK),50％(T1),35％(T2),and 20％(T3)full light were set up.The plant height,stem diameter and the number of main stem segments were measured during the medicinal adult plant stage.The contents of the 3 total components were determined by ultraviolet-visible spectrophotometry.The contents of 4 active components were determined by ultra-performance liquid chromatography,and a comprehensive evaluation was conducted through correlation analysis and entropy weight method.The results show that train T1 had the largest height,which was 54.74 cm,and there was a significant difference from the control group(P＜0.05).The stem diameter of the control group was the largest,at 9.23 mm,which was significantly different from that of T3(P＜0.05),but there was no significant difference from T1 and T2(P＞0.05).There was no significant difference in the number of main stem nodes in each treatment(P＞0.05).With the increase of shading degree,the proportion of leaf dry matter content gradually increases,the proportion of roots dry matter content gradually decreases,and the root-crown ratio gradually decreases.The leaf length,leaf width,leaf area,leaf dry matter content and SPAD value all gradually increase with the increase of shading degree.The contents of each total component and active component showed different variation patterns with the increase of light intensity.The content of total flavonoids gradually increased,while the contents of total phenolic acid,caffeic acid and rosmarinic acid gradually decreased.However,there was no significant difference in the contents of total diterpenoids,charfortin and quercetin in each treatment(P＞0.05).The comprehensive score results of agronomic traits of each treatment obtained by the entropy weight method were T1＞CK＞T2＞T3,and the comprehensive score results of chemical composition were CK＞T1＞T2＞T3.Based on a comprehensive analysis of the agronomic traits and quality indicators,the comprehensive evaluation of agronomic traits at 50％light intensity is the best,and the comprehensive evaluation of chemical components under full light is the best.Therefore,it is suggested that in field production,50％shading treatment should be carried out before sealing,and full light treatment should be carried out during the vigorous growth period after sealing.