多次顶空固相微萃取在不同年份广陈皮挥发性化合物定量中的应用OA

The dried pericarp of Citrus reticulata'Chachi'(GCP,"Guangchenpi"in Chinese)is widely used in traditional Chinese medicine and cuisine.It has been generally recognized that its health benefits are dependent on storage time.As storage years increase,the most relevant components that are volatile oils.However,there are very few reports on the change in the content of volatiles in GCP with the increase of storage years.In this study,we examined the volatiles of GCP based on the headspace solid-phase microextraction method and the gas chromatography-mass spectrometry(HS-SPME-GC-MS).Factors of the extraction duration and temperature,sample size,and the type of fiber coatings were evaluated to quantify the D-Limonene,γ-Terpinene,L-α-Terpineol,and 2-(Methylamino)-benzoic acid-methyl ester in the GCP using the multiple headspace solid-phase microextraction approach(MHS-SPME).A total of 50 volatile compounds were identified,including terpenic,alcohol,aldehyde,and ester series.Results show that the terpenic series in the GCP decays much quicker than the ester and alcohol.The findings in the study shall help discriminate the behavior of the volatile substance during the GCP aging.