山茱萸提取物缓解小鼠急性肾损伤的功能活性评价OA

To investigate the potential mechanism by which Cornus officinalis extract alleviates acute kidney injury,male C57BL/6J mice were selected.An acute kidney injury model was established in mice via intraperitoneal injec-tion of gentamicin.The mice were divided into a normal control group,a model group,and a Cornus officinalis ex-tract intervention group.Body weight,organ coefficients,serum creatinine and urea nitrogen concentrations,and in-flammatory cytokine mass concentrations were measured.Renal histopathological changes were observed by tissue sectioning,and the composition of the intestinal flora was analyzed.The results showed that,compared with the mod-el group,the rate of body weight change was lower in the Cornus officinalis extract group;serum creatinine and urea nitrogen concentrations were significantly reduced;IL-6 and TNF-α mass concentrations were significantly decreased,while IL-10 mass concentration was significantly increased;pathological injuries such as renal tubular epithelial cell necrosis,inflammatory infiltration,and fibrosis were significantly alleviated;the abundance of beneficial bacteria in the intestine increased,while the abundance of harmful bacteria decreased.The results indicate that Cornus officina-lis extract can exert a protective effect on acute kidney injury by regulating inflammatory responses and improving the intestinal microecology.This study elucidates the effects of Cornus officinalis extract on acute kidney injury from his-tological,immunological,and microbiological perspectives,providing a reference for the functional evaluation and precise application of Cornus officinalis extract.