三角鲂胚胎不同发育时期的代谢组分析OA

To investigate the types,dynamic variation patterns and regulatory metabolic pathways of metabolites dur-ing different developmental stages of Megalobrama terminalis embryos,a non-targeted metabolomic approach based on liquid chromatography-mass spectrometry(LC-MS)was employed to analyze embryo samples collected at the gas-trula stage(17 h),neurula stage(25 h),somitogenesis stage(32 h),tail bud stage(41 h),muscular effect stage(50 h)and heart beating stage(58 h).The results showed that a total of 952 metabolites were detected,mainly in-cluding lipids and lipid-like molecules,organic acids and derivatives,and organoheterocyclic compounds.Compara-tive analysis of differential metabolites revealed that 9,11-tetradecadienedioylcarnitine and paraldehyde were unique to Megalobrama terminalis embryos at 32 h of development,while sepiapterin,stearaldehyde and β-nitropropionic acid were specific metabolites at 58 h.KEGG enrichment analysis indicated that metabolic pathways such as nucleo-tide metabolism,ABC transporters and aminoacyl-tRNA biosynthesis exerted important biological functions in the processes of genetic material and protein biosynthesis,energy metabolism and signal transduction during embryonic development of Megalobrama terminalis.The findings of this study can provide theoretical support for improving the hatching rate of fertilized eggs of Megalobrama terminalis.