不同中草药对珍珠龙胆石斑鱼生长性能、抗氧化、消化指标及肠道菌群的影响OACHSSCD

[Objective]To explore the effects of different Chinese herbal medicine feed additives on growth performance,serum physiological and biochemical indexes,intestinal digestive enzyme activity,liver antioxidant immune function and intestinal flora structure of pearl gentian grouper(Epinephelus fuscoguttatus♀×Epinephelus lanceolatus♂),and to provide theoretical basis for the application of Chinese herbal medicine feed additives in the healthy breeding of pearl gentian grouper.[Method]A feeding trial lasting 56 days was conducted using the pearl gentian grouper with initial body mass of(17.64±0.20)g as the research object,and seven treatment groups were set up,including control group,1%(implying mass fraction in feed,the same below)Rabdosiae Rubescentis Herba(Rabdosia rubescens)group,2%R.rubescens group,1%R.rubescens compound group,2%R.rubescens compound group,1%Xiaoyao San group and 2%Xiaoyao San group,with three replicates in each group and 15 fish in each replicate.The growth performance,body shape index,serum physiological and biochemical indexes,intestinal digestive enzyme activity,liver antioxidant and non-specific immune indexes of the fish were measured,and the composition of intestinal flora was analyzed by 16S rRNA high-throughput sequencing.[Result]Different Chinese herbal medicine additives had significant effects on the growth performance,digestive ability,antioxidant immune status and intestinal flora structure of pearl gentian grouper.Compared with the control group,the final average mass,mass gain rate and specific growth rate of 1%R.rubescens group,1%R.rubescens compound group,2%R.rubescens compound group and 1%Xiaoyao San group were significantly increased.Serum biochemical indicators showed that the activities of alanine aminotransferase and aspartate aminotransferase in each Chinese herbal medicine addition group were significantly lower than those in the control group.The results of digestive enzyme showed that the amylase activity of 1%R.rubescens compound group,2%R.rubescens compound group,1%Xiaoyao San group,2%Xiaoyao San group and 2%R.rubescens group was significantly higher than that of 1%R.rubescens group and control group.The pepsin activity of 2%R.rubescens compound group was significantly higher than that of 1%R.rubescens group,2%R.rubescens group,1%Xiaoyao San group and control group.The liver antioxidant and immune indexes showed that the catalase activity of 1%R.rubescens group,2%R.rubescens group and 2%Xiaoyao San group were significantly increased.The activity of superoxide dismutase in 2%R.rubescens compound group and 2%Xiaoyao San group were significantly higher than that in other treatment groups.At the same time,the content of malondialdehyde in the 2%R.rubescens compound group was significantly lower than that in the control group.The analysis of intestinal flora showed that the richness of intestinal flora in the Chinese herbal medicine addition groups were higher than that in the control group,and there were differences in the structure of intestinal flora in each group.At the genus level,the abundance of Lactococcus in Xiaoyao San group was higher,the abundance of Bacillus in R.rubescens group was higher,and the abundance of Vibrio in 1%R.rubescens group,1%R.rubescens compound group,2%R.rubescens compound group,1%Xiaoyao San group and 2%Xiaoyao San group were significantly lower than that in control group.α=0.05.[Conclusion]Under the conditions of this experiment,the comprehensive effect of adding 2%R.rubescens compound Chinese herbal medicine is the best,which can be used as a potential green feed additive to promote the healthy breeding of pearl gentian grouper.