基于代谢组学探讨青盲一号方干预外伤性视神经病变模型大鼠的作用机制OA

OBJECTIVE To explore the mechanism of Qingmang Yihao Formula in intervening in traumatic optic neuropathy(TON)model rats based on metabolomics.METHODS Rats were randomly divided into a control group(CG),a model group(MG),and a Qingmang Yihao Formula group(QM),with six rats in each group.The MG and QM groups underwent establishment of the TON model using the lateral optic nerve traction method,while the CG group only had the optic nerve exposed without traction.The QM group was intragastrically administered 13 g/kg of Qingmang Yihao Formula daily,and the CG and MG groups were administered an equal volume of distilled water,for a continuous administration period of 14 days.After administration,rat retinas were collected.Samples were detected using an ultra-high performance liquid chromatography system and a high-resolution liquid chromatography-mass spectrometer,followed by metabolomic information analysis.RESULTS(1)Metabolite database annotation and functional classification:The classification of metabolites mainly included lipids and lipid-like molecules,organic acids and derivatives,and organic oxygen compounds.The related metabolic pathways primarily covered cancer,neurodegenerative diseases,the digestive system,endocrine system,nervous system,and sensory system.(2)Differential metabolite analysis:A total of 1,121 differential metabolites were detected in rat retinas between the CG and MG groups,with 700 upregulated and 421 downregulated.Between the QM and MG groups,1,153 differential metabolites were detected,with 528 upregulated and 625 downregulated.Further analysis identified 37 differential metabolites.(3)Metabolic pathway analysis:The significantly related metabolic pathways were glycerophospholipid metabolism,arachidonic acid metabolism,and glycosylphosphatidylinositol(GPI)-anchor biosynthesis.While the significance and impact of ether lipid metabolism,steroid hormone biosynthesis,and biosynthesis of unsaturated fatty acids pathways were lower.CONCLUSIONS Qingmang Yihao Formula primarily exerts multiple effects such as anti-inflammatory,antioxidant,stabilization of cell membrane structure,and protection of neural function by regulating glycerophospholipid metabolism,arachidonic acid metabolism,and GPI-anchor biosynthesis,thereby alleviating optic nerve injury and promoting the recovery of visual function.