芷皂烧伤膏对大鼠深Ⅱ度烧烫伤的创面修复作用研究OA

Objective:To investigate the wound repair effect of Zhizao Burn Ointment on deep second degree scalds in rats.Methods:Fifteen SD rats were selected to establish deep second degree scald animal models and randomly divided into 3 groups with 5 rats in each group.The control group was treated with normal saline wet compress on wounds and covered with vaseline gauze stripes,the Zhizao Burn Ointment group and Moist Burn Ointment group were treated with external ointment application respectively for 20 days.Wound healing was ob-served on the day of modeling and the 3rd,7th,10th,14th and 20th day after treatment,and the wound healing rate was calculated.Blood was collected from the tail vein at the 20th day to detect routine blood test,liver and kidney functions.Then the animals were sacrificed,and skin tissue was taken from the center of the burn as the or-igin.Histomorphology was observed after HE staining,and the expressions of Ki67 and Bcl-2 were observed by im-munohistochemistry.Results:From the 7th to 20th day,the unhealed area and wound healing rate in the Zhizao Burn Ointment group were lower than those in the control group(P＜0.01),and were also higher than those in the Moist Burn Ointment group at the 10th day(P＜0.01).From the 7th to 20th day,the eschar area in the Zhizao Burn Ointment group was smaller than that in the control group(P＜0.05),and was also smaller than that in the Moist Burn Ointment group at the 10th day(P＜0.05).At the 20th day,the histological structure of the newborn epidermis in the Zhizao Burn Ointment group was closer to normal skin,with thickness lower than that in the con-trol group and Moist Burn Ointment group(P＜0.01),and inflammatory cell infiltration count lower than that in the control group(P＜0.01).The positive rates of Ki67 cells and Bel-2 cells in the epidermal basal layer in the Zhizao Burn Ointment group and Moist Burn Ointment group were significantly lower than those in the control group(P＜0.05),and the positive rate of Bcl-2 cells in the Zhizao Burn Ointment group was lower than that in the Moist Burn Ointment group(P＜0.05).There were no significant differences in routine blood test,liver and kidney function indexes between the Zhizao Burn Ointment group,Moist Burn Ointment group and the control group(P＞0.05).Conclusion:Zhizao Burn Ointment can promote rapid healing of deep second degree scald wounds,acceler-ate eschar shedding,inhibit skin fibrous tissue hyperplasia after healing and scar formation,with no obvious ad-verse reactions.