鹿角胶通过调节性激素分泌与凋亡蛋白表达对延缓衰老的作用OA

Objective:To investigate the anti-aging effect of Cervi Cornus Colla through regulation of sex hormone secretion and apoptotic protein expression.Methods:Two-month-old rats were designated as the young control group(Group A).Eighteen-month-old male SD rats were randomly divided into three groups:aged control group(Group B),testosterone intervention group(Group C),and Cervi Cornus Colla intervention group(Group D),with 10 rats in each group.The testosterone group received testosterone at a dose of 30 mg/kg per week,while the Cervi Cornus Colla group received 0.6 g·kg-1·d-1 of Cervi Cornus Colla.After 8 weeks of intervention,serum concentrations of gonadotropin-releasing hormone(GnRH),luteinizing hormone(LH),total testosterone(tT),and free testosterone(fT)were measured by ELISA.The levels of apoptosis-related proteins,including p16,p21,Caspase-3,and Ki67,were detected by Western Blot and immunohistochemistry(IHC).Results:The levels of GnRH,LH,tT,and fT in the young control group were significantly higher than those in the other three groups(P＜0.05).The aged control group showed lower GnRH and LH levels compared with the testosterone group(P＜0.05),and significantly lower tT and fT levels than both the testosterone and Cervi Comus Colla groups(P＜0.05).The young control group had significantly lower levels of p16,p21,and Caspase-3 than the other three groups(P＜0.05).The aged control group exhibited significantly higher levels of p1 6,p21,and Caspase-3 compared with the testosterone and Cervi Comus Colla groups(P＜0.05).The testosterone group had significantly lower Caspase-3 level than the Cervi Comus Colla group(P＜0.05).The Ki67 level in the young control group was significantly higher than that in the other three groups(P＜0.05),and the Ki67 level in the testosterone group was significantly higher than that in the aged control group(P＜0.05).Conclusion:Cervi Comus Colla may exert anti-aging effects by regulating sex hormone levels and modulating apoptotic protein expression.