超高效液相色谱-四极杆-静电场轨道阱高分辨质谱法分析蒙药阿格萨-10水提物成分OA

Objective To establish an ultra-high performance liquid chromatography-quadrupole-electrostatic field orbitrap high-resolution mass spectrometry(UPLC-Q-Exactive Orbitrap-MS)method for component analysis of the water extract of Mongolian medicine Agsa-10.Methods The chromatographic column was Waters Acquire UPLC HSS T3 C18 column(100 mm×2.1 mm,1.8 µm),the mobile phase was acetonitrile-0.1%formic acid solution(gradient elution),the flow rate was 0.2 mL/min,the column temperature was 35℃,and the injection volume was 10 µL.The data were collected in positive and negative ion modes by the electric spray ionization(ESI)ion source,and the components were compared and identified according to the retention time,accurate molecular weight,secondary fragment ion information and relevant literature reports.Results Sixty-four chemical components were identified,including seventeen flavonoids,fifteen organic acids,eight terpenoids,eight triterpenoids,five alkaloids,four coumarins,two vitamins,one carbohydrate,one nucleoside,one phthalein,one amide,and one aromatic aldehyde.Conclusion The established method is stable and reliable,which can be used for systematic analysis of the chemical components of the water extract of Agsa-10,and can provide a scientific basis for its subsequent pharmacological substance research and quality standard formulation.