基于双悬臂梁振动结构的谐振式生物组织切片机设计及验证OA

Objective:To design a biological tissue microtome of harmonic vibration type,so as to resolve the issues of conventional rotary microtome,which include big cutting force,and easy damage of fragile tissues.Methods:The vibration structure with dual-cantilever was adopted to design the biological tissue microtome of harmonic vibration type.Based on the kinetic calculation of Euler-Bernoulli equation,the point of harmonic vibration of cantilever of the biological tissue microtome was designed at 55 Hz,so as to realize flexible cutting with high frequency micro amplitude.The control system adopted NE555 time base chip circuit as core to generate the signals of harmonic vibration and feed.Then,the accuracy and precision of the thickness of slice,and the integrity of slice of biological tissue microtome were further verified.Results:The designed biological tissue microtome of harmonic vibration type can reliably realize harmonic vibration at 55 Hz frequency,which resolved the crosstalk of circuit through the strategy of frequency separation.The coefficients of variation were respectively 2.62%and 1.23%when the precision of slice thickness of this system was 3 and 20 μm,which were higher than approximate 30%of conventional microtome.The integrity of slice was favorable when the thicknesses of different type of tissues were between 3 and 20 μm.Conclusion:The biological tissue microtome of harmonic vibration type based on the vibration structure with dual-cantilever can realize stable flexible cutting,which can meet the requirement of the application of biological tissue slices in biomedical research.