梅花鹿产茸性状的全基因组关联分析OA

[Objective]This study conducted genome-wide association study(GWAS)based on whole genome resequencing(WGR)technology to identify candidate genes for antler production traits,providing a theoretical foundation for early precise selection and targeted breeding of high-antler-yield individuals.[Method]Blood samples were collected from 266 Cervus nippon during the antler growth period,and the genomic DNA was subsequently extracted.WGR was performed on the DNBSEQ-T7 platform and GWAS of antler production traits was conducted via the mixed linear model in GEMMA.The associated single nucleotide polymorphism(SNPs)were annotated using ANNOVAR,and further detection was performed via PCR amplification.[Result]A total of 60 317 854 SNPs were identified from Cervus nippon blood samples via WGR.Through GWAS,a total of 67 SNPs significantly associated with the antler-producing trait were identified(P<1×10―6).Based on the results of gene functional annotation,the COMT1,FMNL2,and CRYM genes corresponding to 3 SNPs were further selected for PCR detection,and the results demonstrated that all the SNPs associated with antler production traits within the aforementioned genes exhibited polymorphism.[Conclusion]Based on WGR data,this study identified 67 SNPs potentially associated with the antler production traits of Cervus nippon through GWAS.Among them,COMT1,FMNL2,and CRYM screened out might be important candidate genes affecting the antler production traits.These candidate genes could serve as potential targets for molecular marker-assisted breeding and provide effective molecular markers for analyzing the genetic basis of antler production traits in Cervus nippon.