基于中文数据库的帕金森病动物模型应用分析OA

Objective To summarize the major construction method and phenotypic characteristics of Parkinson's disease(PD)animal models reported in recent Chinese-language databases,with the aim of supporting the refinement of experimental model systems and subsequent mechanistic studies.Methods We systematically searched CNKI,SinoMed,Wanfang,and VIP databases for PD animal-model studies published from January 2022 to August 2025.Data on species,gender age,modelling approach,modelling duration,and assessment method were extracted.A database was built in Microsoft Excel 2024,and inductive analyses were used to organize the application patterns of model types and outcome measures.We supplemented the dataset by extracting traditional Chinese medicine(TCM)-related syndrome information(syndrome label,syndrome judgment items,and syndrome-based modeling)for descriptive summarization.Results A total of 235 studies were included.Rodents predominated,with C57BL/6 mice and Sprague-Dawley rats being most frequently used.Males were usually used,mice were primarily aged 2～4 months,whereas rats were mainly 8～12 weeks old.Fewer studies employed other species such as Drosophila,Caenorhabditis elegans,and non-human primates.Exogenous models comprised neurotoxin induction(e.g.,MPTP,6-OHDA,rotenone),multi-factor combination paradigms,and surgical lesions.Genetic-engineering models included overexpression or mutation of α-synuclein,leucine-rich repeat kinase 2,and PTEN-induced kinase 1.Modelling durations were mainly acute(1～7 days)or chronic(4～8 weeks).Common readouts spanned behavioral performance,histopathology,and molecular assays,focusing on motor deficits,neuroinflammation,mitochondrial dysfunction,and synaptic plasticity.Among studies in which TCM syndromes could be identified(6.81%,16/235),Yin deficiency with internal wind,kidney deficiency,and liver yang hyperactivity were relatively common among TCM syndrome patterns,and the associated diagnostic features included emotional irritability,dry or yellowish fur,and dry or constipated stools.Conclusions The PD animal-model toolbox is diverse.Neurotoxin models remain widely used because of their operational simplicity and reproducibility.Multi-factor induction offers greater physiological relevance for disease progression,while genetic models facilitate the identification of specific pathogenic mechanisms.Future model construction should integrate exogenous and genetic factors,while appropriately incorporating and standardizing the reporting of TCM syndrome elements,and introducing multi-omics and imaging technologies to enhance model stability and translational value,thereby laying a standardized foundation for studies of TCM and other integrated interventions.