LncRNA MIR17HG靶向miR-130a-3p对膀胱癌细胞增殖、迁移和侵袭的影响OA
Impact of LncRNA MIR17HG targeting miR-130a-3p on the proliferation,migration,and invasion of bladder cancer cells
目的 研究长链非编码 RNA 人类微小 RNA17 簇宿主基因(LncRNA MIR17HG)靶向调控微小RNA(miR)-130a-3p 对膀胱癌(BCa)细胞恶性生物学行为的影响.方法 RT-qPCR 法检测人尿路上皮细胞SV-HUC-1和人 BCa 细胞(T24、UM-UC-3、5637)中 LncRNA MIR17HG、miR-130a-3p 的表达.双荧光素酶报告检测 LncRNA MIR17HG 与 miR-130a-3p 之间关系.选取 BCa 细胞 T24 为研究对象,并分为 Con 组、sh-NC 组、sh-LncRNA MIR17HG 组、sh-LncRNA MIR17HG+NC inhibitor 组、sh-LncRNA MIR17HG+miR-130a-3p inhibitor组.RT-qPCR 法检测各组细胞中 LncRNA MIR17HG、miR-130a-3p 水平;采用 CCK8、细胞划痕、Transwell、流式细胞术及 Western blot 实验检测各组细胞增殖、迁移、侵袭、凋亡及相关蛋白水平.结果 在人 BCa 细胞中,LncRNA MIR17HG 水平升高,miR-130a-3p 水平降低(P＜0.05);T24 细胞中两者差异最显著,故选取 T24 细胞进行实验.双荧光素酶报告结果中,WT-LncRNA MIR17HG+miR-130a-3p mimic 组荧光素酶活性相对于 WT-LncRNA MIR17HG+mimic-NC 组较低(P＜0.05).与 Con 组、sh-NC 组比,sh-LncRNA MIR17HG 组 T24 细胞中LncRNA MIR17HG、存活率、侵袭数、划痕愈合率、PCNA、N-cadherin 水平降低,而 miR-130a-3p、凋亡率、cleaved caspase-3、E-cadherin 水平升高(P＜0.05);与 sh-LncRNA MIR17HG 组、sh-LncRNA MIR17HG+NC inhibitor 组比,sh-LncRNA MIR17HG+miR-130a-3p inhibitor 组 T24 细胞中 LncRNA MIR17HG 无明显变化,存活率、侵袭数、划痕愈合率、PCNA、N-cadherin 水平升高,而 miR-130a-3p、凋亡率、cleaved caspase-3、E-cadherin 水平降低(P＜0.05).结论 敲低 LncRNA MIR17HG 的表达可能通过靶向上调 miR-130a-3p 表达,抑制 BCa 细胞的恶性生物学行为.
Objective To study the effect of long noncoding RNA human microRNA17 cluster host gene(LncRNA MIR17HG)on the malignant biological behavior of bladder cancer(BCa)cells by targeting microRNA(miR)-130a-3p.Methods LncRNA MIR17HG and miR-130a-3p were measured in SV-HUC-1 human urinary tract epithelial cells and human BCa cells(T24,UM-UC-3,5637)by reverse transcription-quantitative polymerase chain reaction(RT-qPCR).The relationship between LncRNA MIR17HG and miR-130a-3p was detected by dual-luciferase reporter assay.BCa T24 cells were divided into Control,sh-NC,sh-LncRNA MIR17HG,sh-LncRNA MIR17HG+NC inhibitor,and sh-LncRNA MIR17HG+miR-130a-3p inhibitor groups.LncRNA MIR17HG and miR-130a-3p were detected by RT-qPCR.Cell proliferation,migration,invasion,apoptosis,and related proteins in each group were detected by Cell Counting Kit 8,cell scratch,and Transwell assays,flow cytometry,and Western blot,respectively.Results LncRNA MIR17HG was increased and miR-130a-3p was decreased in human BCa cells(P＜0.05),with the greatest difference in T24 cells.T24 cells were therefore selected for subsequent experiments.Wild-type(WT)LncRNA MIR17HG+miR-130a-3p mimic cells showed lower luciferase activity than WT LncRNA MIR17HG+mimic-NC cells(P＜0.05).In the Control and sh-NC groups,sh-LncRNA MIR17HG cells showed decreases in LncRNA MIR17HG,survival rate,invasion number,scratch-healing rate,proliferating cell nuclear antigen,and N-cadherin in T24 cells,and increases in miR-130a-3p,apoptosis rate,cleaved caspase-3,and E-cadherin(P＜0.05).In the sh-LncRNA MIR17HG and sh-LncRNA MIR17HG+NC inhibitor groups,sh-LncRNA MIR17HG+miR-130a-3p inhibitor cells showed no clear changes in LncRNA MIR17HG in T24 cells,but increases in survival rate,invasion number,scratch-healing rate,PCNA,and N-cadherin,and decreases in miR-130a-3p,apoptosis rate,cleaved caspase-3,and E-cadherin(P＜0.05).Conclusions Knockdown of LncRNA MIR17HG may inhibit the malignant biological behavior of BCa cells by targeting upregulation of miR-130a-3p.
李欣;向威;杨英;孙莹;郑福鑫
武汉市第一医院 泌尿外科,武汉 430000武汉市第一医院 泌尿外科,武汉 430000武汉市第一医院 泌尿外科,武汉 430000武汉市第一医院 泌尿外科,武汉 430000武汉市第一医院 泌尿外科,武汉 430000
医药卫生
长链非编码RNA人类微小RNA17簇宿主基因微小RNA-130a-3p膀胱癌细胞恶性生物学行为
long noncoding RNA human microRNA 17 cluster host genemicroRNA-130a-3pbladder cancercell malignant biological behavior
《中国比较医学杂志》 2026 (10)
9-18,10
国家自然科学基金(81902593).
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