D-半乳糖诱导不同时间对衰老大鼠内皮细胞微粒及氧化应激的影响OA

Objective To investigate the effects of D-galactose induction for different durations on endothelial cell microparticles(EMP)and oxidative stress levels in aging rats.Methods Forty Sprague-Dawley rats were randomly divided into four groups:blank group 1,blank group 2,model group 1 and model group 2.Rats in the model group were injected with D-galactose for 6 and 12 weeks,respectively,to establish an aging rat model.Morphological changes in the thoracic aorta and liver tissues were detected by hematoxylin/eosin(HE)staining.Plasma levels of EMP were measured by flow cytometry.Liver levels of superoxide dismutase(SOD)and malondialdehyde(MDA)and serum interleukin(IL)-6 were measured by enzyme-linked immunosorbent assay.Results After 6 weeks of modelling,plasma levels of CD54+EMP were increased significantly(P＜0.05);SOD liver activity was decreased(P＞0.05),and liver MDA and serum IL-6 levels were showed only an upward trend compared with the blank group 1(P＞0.05);however,the differences were not significant(P＞0.05).HE staining indicated no obvious aging characteristics in the thoracic aorta and liver tissue.After 12 weeks of modelling,however,plasma CD54+EMP levels increased significantly(P＜0.01),SOD liver activity decreased(P＜0.01),MDA levels were substantially elevated(P＜0.01),and serum IL-6 levels were significantly higher compared with the 6-week modelling group(P＜0.01).The thoracic aortic intima was noticeably thickened and the intima/media ratio was significantly increased(P＜0.05).The hepatocyte nuclear volume was also markedly increased,nuclear and cytoplasmic staining deepened,and numerous vacuoles formed,showing obvious signs of aging.Conclusions Induction with D-galactose for 12 weeks can cause significant senescence changes in rats.Concurrently,EMP plasma levels and oxidative stress levels increased with increasing modelling period.In contrast,modelling for 6 weeks result ed in no significant aging characteristics in rats.