广州地区红细胞稀有血型数据库及实体库构建研究OA

Objective To conduct screening for rare blood types within important blood group systems for the Chinese population,such as Rh,Duffy,Kidd,P1Pk,Diego,and MNS,in the Guangzhou region,and to establish a corresponding rare blood type database and physical repository.Methods The saline medium microplate method was used to screen blood donors with the ccDEE phenotype combined with either Jk(a-)or Jk(b-).The polybrene microplate method was employed to screen for donors with Fy(a-),s(-),Lu(b-),Di(b-),k(-),and p phenotypes.The urea lysis microplate method was applied to screen for the Jk(a-b-)phenotype.A high-resolution melting(HRM)curve method was established for screening some donors with the Di(b-)phenotype.Subsequently,expanded phenotyping of antigens in the Rh,Kidd,MNS,Duffy,P1Pk,Lewis,Kell,and Lutheran blood group systems was performed on identified rare blood type donors u-sing monoclonal antibodies.The test results are entered into the Rare Blood Type Bank Management System of the Guang-zhou Blood Center,enabling functions such as confirmation reminders and cryopreservation storage when the donor donates again.Red blood cells of rare blood types are processed into frozen red blood cells for long-term storage.Results Among voluntary blood donors,16 cases of the ccDEE combined with Jk(a-)phenotype were identified(0.221 7%,16/7 216);10 cases of the ccDEE combined with Jk(b-)phenotype(0.138 6%,10/7 216);78 cases of the Fy(a-)phenotype(0.169 5%,78/46 012);39 cases of the Lu(b-)phenotype(0.138 2%,39/28 214);31 cases of the s(-)phenotype(0.081 8%,31/37 913);22 cases of the Di(b-)phenotype(0.029 9%,22/73 691);30 cases of the Jk(a-b-)pheno-type(0.010 1%,30/298 250);and 1 case of the k(-)phenotype(0.001 3%,1/77 382),which was further identified as KELnull phenotype(K0).No p phenotype donors were identified(0/88 528).A total of 228 units of frozen red blood cells were prepared.The screening results were compared and analyzed with rare blood type data from other regions.Con-clusion This study,through a combination of different screening methods,significantly improved the efficiency of rare blood type screening while remaining cost-effective.By conducting large-scale screening and performing data informatization processing,a database and physical repository of rare blood types in the Guangzhou region were successfully established.This provides a strong guarantee for the timely supply of blood to patients with difficult-to-match and rare blood types in the region,effectively enhances the level of transfusion safety in the region,and offers a practical paradigm for constructing a comprehensive blood transfusion support system.