绿茶儿茶素在改善红细胞储存损伤中的作用OA

Objective To evaluate the effects of green tea extract(GTE)and its main catechin monomers on erythro-cyte lesion(such as hemolysis,decreased energy metabolism and oxidative stress)during in vitro storage,and to explore its potential as a novel additive for erythrocyte preservation solutions.Methods The composition of GTE was analyzed by high-performance liquid chromatography(HPLC).Using an in vitro simulated storage model,erythrocytes were stored in CPDA-1 preservation solution supplemented with GTE and the three most abundant catechin monomers(EGCG,ECG,EGC)for 60 days.Hemolysis rate and ATP content were dynamically monitored during storage.Flow cytometry was used to analyze phosphatidylserine(PS)exposure.Meanwhile,the protective effects of each component were verified in an acute oxidative stress model,and erythrocyte membrane stability was assessed by osmotic fragility test.Results After 60 days of storage at 4℃,the hemolysis rate at the end of storage in the GTE group was<0.8%,which was superior to that in the control group and the single catechin-supplemented groups.Erythrocyte osmotic fragility assay showed that GTE could en-hance the stability of erythrocyte membranes.In the acute oxidative stress experiment,the protective rate of GTE against e-rythrocyte injury exceeded 99%,which was better than that of the single catechin groups.At the initial stage of storage,ATP content decreased in all catechin-treated groups,but PS exposure was not significantly increased.Conclusion The addition of GTE can effectively alleviate storage lesions of erythrocytes,with efficacy superior to that of single catechins.GTE enhances the antioxidant capacity and membrane stability of stored erythrocytes.Our results provide new experimental evidence for the development of GTE-based erythrocyte preservation additives.