基于荧光SSR标记的杧果杂交后代杂种真实性鉴定OA

Hybrid breeding is the most important and effective method for developing new mango varieties,and the rapid and efficient identification of hybrid progeny is a prerequisite for the implementation of breeding programs.In this study,the hybrid progeny and their parents from three crosses including'Jinhuang'×'Renong No.1','Jinhuang'×'Irwin',and'Renong No.1'×'Tainong No.1'were used as experimental materials.With capillary electrophoresis,fluorescent polymorphic SSR markers were screened between the parents and further used to identify their hybrid progeny.The results showed that seven in-dividuals of the'Jinhuang'×'Renong No.1'cross at seven loci,six individuals of the'Jinhuang'×'Irwin'cross at six lo-ci,and eleven individuals of the'Renong No.1'×'Tainong No.1'cross at four loci amplified polymorphic bands from both parents,indicating all of them were hybrids.In the UPGMA clustering analysis,141132 and 141193 were clustered with'Jin-huang',1411100,130405,and 141166 were clustered with'Renong No.1',while 141182 and 141195 were clustered in a sep-arate category.All F1 individuals from both'Jinhuang'×'Irwin'and'Renong No.1'×'Tainong No.1'crosses were clustered with their respective paternal parents,showing a tendency of paternal inheritance.The results of this study provide important theoretical basis and reference for the identification of mango hybrids using fluorescent SSR molecular markers.