富民枳组培育苗及光合功能分析OA

To protect and utilize the germplasm resources of Poncirus polyandra,its young stem segments were used as explants to establish an in vitro regeneration system,and the photosynthetic function of the tissue-cultured seedlings was com-pared with that of seed-grown seedlings.The results showed that the optimal medium for axillary bud induction was MT+6-BA 2.0 mg/L+IAA 0.2 mg/L+casein hydrolysate(CH)200 mg/L,with an induction rate of 88.9％and vigorous growth.The optimal medium for adventitious bud proliferation was WPM+6-BA 0.5 mg/L+IAA 0.5 mg/L+activated carbon(AC)0.2 g/L,with a proliferation coefficient of 8.7.The optimal medium for root induction was MT+6-BA 1.0 mg/L+IBA 0.5 mg/L+powdered activated carbon(PAC)0.2 g/L,with a rooting rate of 73.33％and a rooting coefficient of 5.85.The transpiration rate and chlorophyll content of tissue-cultured seedling leaves were both significantly higher than those of the seed-grown seedlings.The photosynthetic function of tissue-cultured seedlings was superior to that of seed-grown seedlings,but their tolerance to drought was reduced.Therefore,attention should be paid to strengthening field drought-resistance management.