基于细胞学评价的鱼油抗炎活性研究OACHSSCD

Aims:In view of the inconsistent anti-inflammatory activities of commercial fish oil products and the lack of a unified evaluation method,this study aims to establish an in vitro evaluation system for the anti-inflammatory activity of fish oil based on HepG2 and Caco-2 cell inflammation models,so as to provide a basis for the objective comparison of product efficacy and quality control.Methods:Optimal concentrations of isopropanol,fish oil,and lipopolysaccharide(LPS)were selected to establish LPS-induced inflammatory models in HepG2 and Caco-2 cells.The applicability of the established models for evaluating the anti-inflammatory activity of fish oil was verified using realtime quantitative polymerase chain reaction(RTqPCR)and enzymelinked immunosorbent assay(ELISA);and the antiinflammatory activities of 10 commercial fish oil products were subsequently evaluated.Results:Isopropanol(≤2.5％)and fish oil(≤900μg/mL)exhibited no inhibitory effects on cell viability.Lipopolysaccharide(LPS)at 1 μg/mL and 3 μg/mL was selected for establishing inflammatory models in HepG2 and Caco-2 cells,respectively.Compared with RTqPCR,which reflected dynamic transcriptional levels,the ELISA method showed superior linearity and stability in characterizing the doseinhibitory effect relationship of fish oil.The linear correlation coefficient R2 was greater than 0.98.The intra and interassay precision coefficients of variation were less than 15％;and the stability CV was less than 15％,all of which met the methodological requirements.The established model was applied to evaluate 10 commercially available fish oil products.The results demonstrated significant heterogeneity in antiinflammatory activities among different products,among which specific products(YY-4,YY-6)exhibited strong antiinflammatory inhibitory effects in both cell models.Conclusions:The established standardized in vitro evaluation system with ELISA as the core detection method is suitable for evaluating the anti-inflammatory activity of fish oil products.It can provide reliable technical support for the functional evaluation and quality grading of fish oil products.