橙色荧光碳点的合成及其对过氧亚硝酸盐的荧光/比色双模式检测OA
Synthesis of Orange Fluorescent Carbon Dots for Fluorescence/Colorimetric Dual-Mode Detection of Peroxynitrite
过氧亚硝酸盐(ONOO-)在细胞信号转导及体内平衡调节中发挥着重要作用,实现ONOO-的检测对疾病的早期诊断和治疗至关重要.本文以罗丹明B和对氨基苯酚为前体,采用一步溶剂热法成功制备了橙色荧光碳点(O-CDs).所制备的O-CDs因表面的氨基/羟基官能团可被ONOO-氧化,对其表现出良好的选择性和荧光猝灭响应.当ONOO-浓度处于30～480和670～1070 nmol/L范围时,其浓度与荧光强度变化的线性相关性良好,检出限为9 nmol/L.与此同时,随着ONOO-浓度变化,溶液颜色由粉红色逐渐变为深蓝色,吸光度与ONOO-浓度在300～1000 nmol/L范围内呈良好线性响应,检出限为90 nmol/L.因此,该工作成功实现了对ONOO-的荧光和比色双模式的检测.此外,凭借其优异的光稳定性及低毒性等特性,O-CDs成功应用于PC-12活细胞中ONOO-的荧光成像,这为阐释ONOO-在相关病理过程中复杂的生物学作用提供了重要手段.
Peroxynitrite(ONOO-)plays an important role in cellular signal transduction and homeostasis;thereby,achieving the detection of ONOO-is crucial for early diagnosis and treatment of diseases.Herein,orange fluorescent carbon dots(O-CDs)were successfully synthesized by a one-step solvothermal method using rhodamine B and p-aminophenol as precursors.The O-CDs exhibited good selectivity and a sensitive response to ONOO-due to the oxidation of the amino/hydroxyl functional groups on their surface by ONOO-.Within the concentration ranges of 30～480 and 670～1070 nmol/L,the quenching efficiency of O-CDs showed a good linear correlation with the concentration of ONOO-,with the detection limit of 9 nmol/L.Meanwhile,the color of the solution gradually changed from pink red to dark blue.For the colorimetric mode,a good linear relationship between the absorbance change and ONOO-concentration was obtained in the range of 300～1000 nmol/L,with the detection limit of 90 nmol/L.Consequently,this work has achieved the fluorescent and colorimetric dual-mode specific recognition and detection of ONOO-.In addition,due to the excellent photostability and low toxicity of O-CDs,they have been successfully applied for the fluorescent imaging of ONOO-in PC-12 living cells,providing a valuable tool for elucidating the complex biological roles of ONOO-in related pathological processes.
郭建花;王松柏;邢晋羚;双少敏;董川
山西电子科技学院环境与生命科学学院,临汾 041000||山西大学化学化工学院,环境科学研究所,太原 030006山西大学化学化工学院,环境科学研究所,太原 030006山西大学化学化工学院,环境科学研究所,太原 030006山西大学化学化工学院,环境科学研究所,太原 030006山西大学化学化工学院,环境科学研究所,太原 030006
化学化工
橙色荧光碳点荧光和比色过氧亚硝酸盐细胞成像
Orange fluorescent carbon dotsFluorescent and colorimetricPeroxynitriteCellular imaging
《应用化学》 2026 (5)
719-728,10
山西电子科技学院科研启动经费(No.2025KJ007)、山西省高等学校科技创新项目经费(No.2025L168)和国家自然科学基金(No.22274090)资助 Supported by the Startup Fund of Shanxi University of Electronic Science and Technology(No.2025KJ007),the Science and Technology Innovation Project Funding of Colleges and Universities in Shanxi Province(No.2025L168)and the National Natural Science Foundation of China(No.22274090)
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