基于生物信息学分析糖尿病肾病肾小球自噬相关基因OA

Objective To explore the biomarkers,mechanisms and potential drugs of autophagy in diabetic nephropathy based on bioinformatics technology.Methods The GSE1009 dataset was downloaded to identify differentially expressed genes in diabetic nephropathy,and autophagy-related genes were downloaded from the Human Autophagy Database.The intersection of autophagy-related genes and differentially expressed genes in diabetic nephropathy was taken to obtain the common genes.GO and KEGG enrichment analyses were performed on diabetic nephropathy autophagy-related genes(DE-ATGs).In addition,PPI network was used to identify the top 10 hub genes of the DE-ATGs.The expression profiles of the hub genes were validated using the GSE96804 dataset.Clinical correlation analysis was performed using Nephroseqv5.Potential targeted drugs for the hub genes were predicted using The DrugGene Interaction Database.Results A total of 1244 differentially expressed genes in diabetic nephropathy were screened from the GSE1009 dataset,and 232 autophagy-related genes were downloaded from the Human Autophagy Database database,and 22 DE-ATGs were obtained by intersection of the two.DE-ATGs biological processes were significantly enriched in regulation of autophagy,autophagy of mitochondrion.KEGG enrichment analysis significantly enriched in Autophagy-animal,Longevity regulating pathway,Autophagy-other and other signaling pathways.The top 10 core genes screened by PPI protein interaction network were VEGFA,HSPA5,ATG5,MYC,CASP1,EIF4EBP1,BAX,IFNG,RHEB and ATG4B.VEGFA was positively correlated with glomerular filtration rate,HSPA5 and CASP1 were negatively correlated with glomerular filtration rate.Targeted drugs or ingredients including FENOFIBRATE,ENALAPRIL,SILDENAFIL may up-regulate the expression level of VEGFA,and DAPHNETIN can up-regulate the expression level of HSPA5.BELNACASAN and DIACEREIN can inhibit the expression of CASP1.Conclusion VEGFA,HSPA5 and CASP1 may be new markers for the diagnosis and evaluation of diabetic nephropathy.Drug-gene interaction analysis provides a possible candidate drug for the treatment of diabetic nephropathy.