圆口铜鱼养殖群体和野生群体遗传多样性比较分析OA

[Objective]By comparing the differences in genetic diversity between cultured and wild populations of Coreius guichenoti,this study aimed to provide a theoretical basis and technical reference for the artificial breeding and release activities.[Method]A total of 100 samples were collected from one wild population and two cultured populations.Population genetic analysis was conducted using microsatellite molecular markers.[Result]A total of 205 alleles were detected across 15 microsatellite markers.All three populations of C.guichenoti exhib-ited high genetic diversity(Ho=0.630-0.742,He=0.687-0.787).Genetic differentiation and molecular analysis of variance revealed that the cultured populations A1 and A2 were moderately differentiated(0.05＜Fst＜0.15);genetic differentiation among the remaining populations was low.Approximately 92.89％of the genetic variation in C.guichenoti originated within populations,indicating that individual variation was the primary source of genetic diversity.Clustering analysis based on the UPGMA method indicated that the wild population(W)was the most closely related to cultured population(A1),while the two cultured populations were the most genetically distant.This finding was in agreement with the principal coordinate analysis(PCoA).[Conclusion]The wild population of C.guichenoti exhibite higher genetic diversity than the cultured populations.It is recommended to update the broodstocks and select individuals with greater genetic distance for artificial breeding to improve the genetic diversity of released juveniles.Additionally,continuous release activities should be avoided to pre-vent alterations in the genetic composition of C.guichenoti.