活筋壮骨散对大鼠骨关节炎的治疗作用研究OA

Osteoarthritis(OA)is a common chronic disease,characterized mainly by cartilage degeneration,joint pain,inflammation,and gradual loss of joint function.Huojin Zhuanggu San(HJZGS),a traditional Chinese medicine compound,composed of six herbal ingredi-ents,was evaluated in this study for its therapeutic effects and potential mechanisms of action on OA using an OA rat model.In vivo,the OA rat model was established by intra-articular injection of sodium monoiodoacetate.After 21 days of oral administration of HJZGS,the thera-peutic effects were assessed by histological observation and measurement of serum cytokine levels.In vitro,inflammation was induced in RAW 264.7 cells using LPS.The polarization of macrophages,mitochondrial membrane potential,reactive oxygen species(ROS)produc-tion,cell cycle and apoptosis were detected after HJZGS intervention to evaluate the anti-inflammatory ability of HJZGS.The in vivo study demonstrated that HJZGS significantly alleviated joint cartilage damage in OA rats and reduced the levels of pro-inflammatory cytokines such as IL-6 and TNF-α in serum.The in vitro study showed that HJZGS modulated macrophages polarization,promoting the shift of macrophages from the M1 to the M2 phenotype,thereby exerting anti-inflammatory effects.Additionally,HJZGS reduced the production of ROS,en-hanced cellular antioxidant capacity,and rescued mitochondrial membrane potential,thereby minimizing cell damage.HJZGS effectively alle-viated sodium monoiodoacetate-induced OA in rats by modulating macrophages polarization and inhibiting inflammation and oxidative stress.This finding provided experimental evidence for application of HJZGS in veterinary treatment of canine OA and laid a theoretical foundation for development of functional pet food additives.