母鸡产蛋后期贮精能力群体变异分析及高低贮精能力组代谢特征比较OA
Analysis of Population Variation of Hen's Sperm Storage Capacity in Late Laying Period and Comparison of Metabolic Characteristics between High and Low Sperm Storage Capacity Groups
旨在解析白来航蛋鸡产蛋后期贮精能力的群体变异,对高低贮精能力组代谢特征进行比较.本研究选取1 100只90周龄白来航蛋鸡,记录受精率、持续受精天数等表型;基于表型(产蛋率＞75％)筛选高、低受精率个体各10只,分别定义为高、低贮精能力组,分离血清检测雌二醇(estradiol,E2)、孕酮(progesterone,P4)、前列腺素F2α(prostaglandinF2α,PGF2α)和睾酮(testosterone,T)水平;同时利用血清非靶向代谢组筛选高、低贮精能力组间差异代谢物,并进行KEGG通路富集分析.结果显示,产蛋后期母鸡群体的各项贮精能力指标均表现出较大的个体间变异,其中,间歇式受精强度和最大连产受精蛋数变异系数较高,分别为95.57％和52.76％;血清T和PGF2α水平在高贮精能力组水平更高(P＜0.05);鉴定出58种差异代谢物,其中25种在高贮精能力组表达上调,33种表达下调;KEGG富集分析表明贮精能力与脂质代谢通路高度相关;同时,高贮精能力组血清二酮古洛酸、24-亚甲基胆固醇等显著上调与其抗氧化能力密切相关.综上,高贮精能力组具备更强的抗氧化能力与脂质代谢活性,从而防止脂质过氧化对精子的损伤,以此提升母鸡的贮精能力.
The purpose of this study was to analyze the population variation analysis of sperm storage capacity and compare the metabolic characteristics of high and low sperm storage capacity groups in the late laying period of White Leghorns laying hen.A total of 1 100 90-week-old White Leghorns laying hens were selected,and the phenotypes such as fertility rate and effective fertilization days were recorded.Based on the phenotype(egg laying rate＞75％),10 individuals with high fertility rate and 10 individuals with low fertility rate were selected,which were respectively defined as high and low sperm storage capacity groups.Serum was separated to detect the levels of estradiol(E2),progesterone(P4),prostaglandin F2α(PGF2α)and testosterone(T).At the same time,the differential metabolites between high and low sperm storage capacity groups were screened by serum non-targeted metabonomics,and KEGG pathway enrichment analysis was carried out.The results showed that the indexes of sperm storage capacity of hen population showed great individual variation in the late laying period,among which the coefficient of variation of intermittent fertilization intensity and the maximum number of consecutive fertile eggs were higher,which were 95.57％and 52.76％respectively.The levels of serum T and PGF2α were higher in the group with high sperm storage capacity(P＜0.05).A total of 58 differential metabolites were identified,of which 25 were up-regulated and 33 were down-regulated in the group with high sperm storage capacity.KEGG enrichment analysis showed that sperm storage capacity was highly correlated with lipid metabolism pathway.At the same time,the significant increase of serum diketone gulonic acid and 24-methylene cholesterol in the group with high sperm storage capacity is closely related to its antioxidant capacity.In conclusion,high sperm storage capacity group has stronger antioxidant capacity and lipid metabolism activity,thus preventing the damage of lipid peroxidation to sperm,and enhancing the sperm storage capacity.
徐俊杰;邸科前;陈辉;石雷;李欣欣;李艳慧;范毅豪;吴星霖;王德贺;陈一凡;郝二英;赵晓钰
河北农业大学动物科技学院,保定 071001河北大学基础医学院,保定 071002河北农业大学动物科技学院,保定 071001河北农业大学动物科技学院,保定 071001河北农业大学动物科技学院,保定 071001河北农业大学动物科技学院,保定 071001河北农业大学动物科技学院,保定 071001河北农业大学动物科技学院,保定 071001河北农业大学动物科技学院,保定 071001河北农业大学动物科技学院,保定 071001河北农业大学动物科技学院,保定 071001保定兴芮农牧发展有限公司,保定 072550
农业科技
母鸡贮精能力群体变异激素水平代谢组学
hensperm storage capacitypopulation variationhormone levelmetabolomics
《畜牧兽医学报》 2026 (5)
2625-2635,11
河北省现代农业产业技术体系建设项目(HBCT2024260201)保定市"揭榜挂帅"项目(2024农1012024农206)河北省省级科技计划(22326605D)
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