基于微卫星标记分析同羊种公羊的遗传多样性OA

[Objective]This study aims to assess the genetic diversity of breeding rams of the Tong sheep breed using microsatellite markers,in order to provide a scientific basis for pedigree identification and genetic management in Tong sheep farming.[Method]A total of 36 rams of the Tong sheep breed were selected for analysis.Genomic DNA was extracted from blood samples of the rams,and PCR amplification was performed using nine fluorescently labeled microsatellite primers,including one Y-chromosome-specific marker(SRYM18)and eight autosomal markers(MAF65,AE129,ILSTS5,JMP29,SRCRSP9,FCB304,MCM140,BM8125).Genotyping analysis was completed by using CERVUS software.Nei's genetic distances between individuals were calculated by using the Poppr package in R 4.4.1 software,and a systematic cluster diagram was con-structed by using the UPGMA method to analyze the genetic diversity characteristics of Tong sheep rams.[Re-sult]Except for the Y-linked locus SRYM18,all the other eight autosomal microsatellite loci exhibited high polymorphism.The cumulative exclusion probability for single-parent exclusion was 95.27%when both paren-tal genotypes were unknown,the cumulative exclusion probability for single-parent exclusion was 99.54%when one parental genotype was known,and the cumulative exclusion probability for both-parent exclusion was 99.99%when both parental genotypes were unknown.The 36 rams were classified into nine distinct paternal lineages based on UPGMA clustering.[Conclusion]This study successfully identified and categorized the pedi-grees of Tong sheep rams,providing a scientific foundation for ram selection and management,thereby promo-ting the sustainable development and genetic improvement of Tong sheep breeding programs.