槲皮素对苯并芘诱导人肺腺癌A549细胞损伤的保护作用OA

Objective To investigate the protective effect of quercetin against benzo(a)pyrene(BaP)-induced damage in hu-man lung adenocarcinoma A549 cells by regulating Drp1 expression to improve mitophagy.Methods The A549 cells were randomly divided into 4 groups:a control group(normal culture),a BaP group(treated with BaP),a BaP+Quercetin group(quercetin inter-vention+BaP treatment),and a BaP+Mdivi-1 group(Drp1 inhibitor Mdivi-1+BaP treatment).Cell viability was measured by CCK8 assay,apoptosis was observed by TUNEL staining,cell volume was observed by phalloidin staining,changes in organelles were exam-ined by transmission electron microscopy,and the expression levels of autophagy-related proteins(Drp1,PINK1,Parkin)was ana-lyzed by Western blotting.Results Compared with the Control group,the BaP group showed reduced cell viability(P<0.05),in-creased apoptosis rate and elevated expression of autophagy-related proteins(Drp1,PINK1,Parkin)increased(P<0.05).Compared with the BaP group,the cell viability in the BaP+Quercetin group and the BaP+Mdivi-1 group increased(P<0.05),and the apoptosis rate and the expression of autophagy-related proteins(Drp1,PINK1,Parkin)decreased(P<0.05).Conclusions Quercetin can regu-late the expression of Drp1 to improve abnormal mitophagy and achieve the protective effect on A549 cells damage induced by BaP.