川芎和当归挥发性成分指纹图谱与多成分定量分析及差异比较OA

[Objective]To establish an Ultra Performance Convergence Chromatography(UPC2)fingerprint and multi-component content determination method for the volatile components of Ligusticum chuanxiong and Angelica sinensis,and to compare the differences in volatile components between the two herbs.[Methods]Supercritical fluid chromatography(SFC)was used to extract the volatile components from L.chuanxiong and A.sinensis using CO2 as the mobile phase and methanol as a modifier.A Waters Viridis® BEH column(3.0×100 mm,130 Å,1.7 µm)was employed to establish the fingerprint and content determination methods,with CO2 as the mobile phase and 0.1%formic acid in methanol(v/v)as the modifier.Chemical pattern recognition was conducted on the common peaks of the two herbs to identify the key differential components.[Results]Eighteen common peaks were identified in 11 batches of L.chuanxiong,with a similarity greater than 0.94.Eleven common peaks were identified in 11 batches of A.sinensis,with a similarity greater than 0.96.Seven major common peaks were identified by reference standards,namely ligustilide,senkyunolide A,levistolide A,ferulic acid,senkyunolide H,coniferyl ferulate,and senkyunolide I.The contents of these seven chemical components in L.chuanxiong were higher than those in A.sinensis,among which the key differential components were ligustilide,senkyunolide A,and coniferyl ferulate.[Conclusion]The UPC2 fingerprint and multi-component quantitative methods for L.chuanxiong and A.sinensis were demonstrated to be rapid,stable,reliable,and environmentally friendly,which can serve as a reference for improving the quality control standards of these two herbs.The lower content of volatile components in A.sinensis than in L.chuanxiong may be the material basis for their differences in efficacy.