木犀草素联合白杨素应用对人肾癌细胞A498凋亡的影响OA

This study aimed to investigate the effect of combined chrysin and luteolin on the apoptosis of human re-nal cell carcinoma cell A498.Human renal cell carcinoma A498 cells in the logarithmic growth phase were harves-ted.The experiment was divided into four groups:control group,luteolin group,chrysin group,and chrysin-luteolin combination group.The CCK-8 assay was used to determine the effects of chrysin,luteolin,and their combination on the survival rate of A498 cells.Laser scanning confocal microscopy(CLSM)was employed to measure intracellu-lar reactive oxygen species(ROS)levels and changes in mitochondrial membrane potential,while flow cytometry was used to detect cell apoptosis.When the concentration of luteolin is 40 μmol/L,the survival rate of A498 cells is 79.28%;when the concentration of poplar extract was 40 μmol/L,the cell survival rate of A498 was 75.32%;when 40 μmol/L luteolin was combined with 40 μmol/L chrysin,the cell A498 survival rate was further reduced to 74.81%.After the combination of the two drugs,the intracellular reactive oxygen species level was 3.4 times higher than the control group,and the mitochondrial membrane potential decreased significantly.The apoptosis rate of A498 cells significantly increased after the combination application.The combined application of luteolin and poplar ex-tract has a significant inhibitory effect on the proliferation and apoptosis rate of human renal cancer cell line A498.