朝鲜淫羊藿不同部位化学成分及其与抗氧化活性相关性研究OA

Objective To determine the contents of seven flavonoid constituents and total flavonoids in different parts of Epimedium koreanum Nakai and to investigate their antioxidant activity,so as to provide references for quality control and resource utilization.Meth-ods The seven flavonoid constituents in the leaves,petioles,rachises,and stems of Epimedium koreanum were quantified using high-performance liquid chromatography(HPLC).Differences among plant parts were analyzed via chemometrics.Total flavonoid content was determined by UV-Vis spectrophotometry.Antioxidant activity was evaluated by measuring the scavenging rates against 1,1-diphe-nyl-2-picrylhydrazyl(DPPH)radicals,hydroxyl radicals,and superoxide anion radicals.Results The total content of the seven flavo-noids in different parts ranked in the following order:leaves>petioles>rachises>stems.The leaf samples exhibited significantly higher levels of the seven specific flavonoids,total flavonoids,and antioxidant activity compared to the petiole,rachis,and stem samples.Conclusion This study revealed that the leaves of Epimedium koreanum possess superior levels of the analyzed flavonoids,total flavonoids,and antioxidant activity compared to its petioles,rachises,and stems.These findings provide a basis for the quality control and resource utilization of this medicinal plant.