两种诱导子对党参不定根生长及多糖含量的影响OA

Objective To clarify the growth curve of the suspension culture of Codonopsis pilosula adventitious roots and to investigate the effects of two inducers,methyl jasmonate(MeJa)and chitosan(CTS),on the growth and polysaccharide content of the adventitious roots.Methods The dry and fresh weights of C.pilosula adventitious roots were measured at different days after inoculation.Two differ-ent types of inducers with varying concentrations were added to the adventitious root suspension culture system.Results The growth curve of C.pilosula adventitious roots showed an"S"shape.The optimal concentration of MeJa was 150 μmol/L with a suitable induc-tion time of 8 days;the optimal concentration of CTS was 50 mg/L with a suitable induction time of 4 days.Conclusions In the suspen-sion culture of C.pilosula adventitious roots,adding an appropriate concentration of MeJa or CTS on the 15th day of culture and induc-ing for a suitable duration can significantly increase the polysaccharide content of the adventitious roots.