乌梅丸改善肥胖小鼠血管内皮功能的作用机制研究OA

Objective To investigate the effects and mechanisms of Wumei Pill(乌梅丸,WMP)on endothelial function in obese mice and its impact on oxidized low-density lipoprotein(ox-LDL)-induced endothelial injury.Methods A high-fat diet(HFD)-induced obe-sity mouse model was established by feeding the mice for 24 weeks.Afterwards,the successfully established obese mice were randomly divided into the following groups:model group,the three WMP-treated groups at low,medium and high doses,and metformin group.The control group and model group were administered normal saline via gavage,while the other groups received the corresponding drug treatments via gavage for an additional 7 weeks.Body weight was recorded,using HE staining to observe morphological changes in the aorta,detecting serum lipid levels,and employing ELISA to measure inflammatory cytokine levels in each group.In vitro,HUVECs were cultured,and the proliferation,apoptosis,tubule formation,migration,and transwell migration abilities of the cells were assessed using CCK-8 assay,TUNEL assay,tubule formation assay,scratch assay,and transwell assay,respectively.Results Compared with the model group,the high-dose WMP group showed significant decrease in body weight,serum lipids,and malondialdehyde levels(P<0.05),while catalase activity was increased(P<0.01).Additionally,the levels of interleukin-1β and interleukin-6 were significantly reduced(P<0.001).HE staining revealed improved morphological changes in the aortic tissue.Compared to the ox-LDL group,the 10%WMP group showed enhanced cell proliferation and migration capacity(P<0.05)and a significant reduction in apoptosis(P<0.001).Conclusion WMP can improve endothelial function in obese mice and alleviate ox-LDL-induced endothelial cell injury,with its mechanism possibly involving reducing inflammation,antioxidant effects,promoting endothelial cell proliferation and migration,and inhibiting cell apoptosis.