补肾活血方调控Th17分化偏移治疗不明原因复发性流产的作用机制研究OA

Objective To explore the mechanism of Bushen Huoxue Formula(补肾活血方,BSHXF)in treating unexplained recurrent spontaneous abortion(URSA)by regulating the bias of Th17 cell differentiation.Methods Normal pregnancy mouse models were estab-lished by mating CBA/J female mice with BABL/c male mice,while URSA pregnancy mouse models were established by mating CBA/J female mice with DBA/2 male mice.The normal pregnant mice served as the blank control group.The URSA pregnant mice were ran-domly divided into the model group,the BSHXF group,and the Western medicine group,with 15 mice in each group.All pregnant mice were gavaged from the first day of vaginal plug detection for 14 consecutive days(the control group and the model group were given the same volume of saline,the BSHXF group,and the Western medicine group,with 15 mice in each group.Intragastric administration began on the first day a vaginal plug was detected(the control and model groups received the same volume of normal saline,the Chinese medicine group received the aqueous decoction of BSHXF,and the Western medicine group received dydrogesterone)and continued for 14 days.On the last day,the mice were sacrificed and samples were collected 2 hours after administration.The embryo absorption rate of each group was observed.Real-time PCR and Western blot(WB)were used to detect the protein and mRNA expression levels of RORγt,IL-17,and IL-22 in the decidual tissue,and ELISA was used to measure the serum levels of IL-17 and IL-22 in mice from each group.Results Compared with the control group,the model group showed a significant increase in the embryo absorption rate(P<0.05),significantly elevated serum IL-17 levels(P<0.05),significantly increased IL-22 levels(P<0.01),and significantly higher protein and mRNA expression of RORγt,IL-17,and IL-22 in the decidual tissue(P<0.01).Compared with the model group,the BSHXF group could significantly reduced the embryo absorption rate(P<0.05)and significantly downregulated the protein and mRNA levels of RORγt,IL-17,and IL-22(P<0.01).There was no significant statistical difference between the BSHXF group and the West-ern medicine group(P>0.05).Conclusion Th17 cell differentiation is biased in URSA pregnant mice.BSHXF can reduce the levels of the related transcription factor RORγt and inflammatory factors IL-17 and IL-22,reverse the bias of Th17 cell differentiation,decrease the embryo absorption rate,and improve the pregnancy outcome in URSA pregnant mice.