半夏泻心汤调控p53/SAT1/ALOX15通路干预胃癌前病变的研究OA

Objective Expiloring the effect of Banxia Xiexin Decoction(半夏泻心汤,BXXXD)on regulating the p53/SAT1/ALOX15 pathway in rat gastic tissue and intervening in gastric precancerous lesions(GPL).Methods Twenty-four SD rats were randomly divided into a control group,a model group,and a BXXXD group.The control group received standard feeding,while the other two groups were modeled using the N-methyl-N'-nitro-N-nitrosoguanidine(MNNG)multi-factor composite method.After 19 weeks of continuous modeling,the model group and the BXXXD group continued to have free access to MNNG solution.Additionally,the BXXXD group received daily intragastric administration of BXXXD at a dose of 1 mL/100 g for 10 consecutive weeks.Gastric muco-sal histopathological changes were observed under light microscopy using HE staining.The mRNA expression levels of p53,SAT1,and ALOX15 were detected by RT-PCR.The protein expression levels of p53,SAT1,and ALOX15 were determined by Western blot(WB).Results HE staining revealed moderate to severe mucosal atrophy with some dysplasia in the model group,while the BXXXD group exhibited only mild atrophy and no dysplasia.Compared to the control group,the mRNA expression levels of p53,SAT1,and ALOX15 were significantly decreased in the model group(P<0.01).BXXXD intervention significantly increased the mRNA expres-sion levels of p53,SAT1,and ALOX15 compared to the model group(P<0.01).Similarly,the protein expression levels of p53,SAT1,and ALOX15 were significantly lower in the model group than in the control group(P<0.01),and these levels were higher after BXXXD intervention compared to the model group(P<0.01).Conclusion BXXXD may alleviate the pathological state of GPL in rats by regulating ferroptosis through the p53/SAT1/ALOX15 pathway.