肉苁蓉总苷对MPTP诱导的帕金森病模型小鼠的神经保护作用OA

Objective:To explore the protective effects and dose-effect relationship of glycosides of cistanche(GCs)on dopamine(DA)neurons in the substantia nigra of Parkinson's disease(PD)model mice.Methods:Sixty male C57BL/6 mice were randomly divided into the control group,model group,GCs administration groups(50,100,200 mg/kg)and positive control group,with 10 mice in each group.Except for the control group,all other groups were intraperitoneally injected with MPTP to establish the PD model.The GCs groups and the positive control group were administered corresponding doses by intragastric administration.Behavioral tests were performed to evaluate the motor function of mice.Immunohistochemical staining and Western blot were used to detect the number of tyrosine hydroxylase(TH)-positive neurons and TH protein expression level in the substantia nigra pars compacta of the midbrain,respectively.The DA content in the striatum was determined by ELISA.Results:Compared with the control group,the model group presented decreased motor ability(P＜0.01),reduced TH-positive cells and TH protein expression in the substantia nigra pars compacta of the midbrain(P＜0.01),and decreased DA content in the striatum(P＜0.01).Compared with the model group,the 100 mg/kg GCs group significantly improved the above indicators(P＜0.05),and the effect was better than that of the 50 and 200 mg/kg GCs groups(P＜0.05).Conclusion:GCs have a neuroprotective effect on DA neurons in PD model mice,with an optimal therapeutic effect at the dose of 100 mg/kg.