粉尘螨可培养共生细菌的分离、鉴定与多样性分析OA

Objective:To clarify the composition of culturable symbiotic bacteria in Dermatophagoides farinae,and analyze their composition characteristics and diversity levels.Methods:Bacterial isolation and culture methods were used to isolate and purify culturable symbiotic bacteria from laboratory-reared Dermatophagoides farinae.The isolated and purified strains were identified through morphological characteristics,Gram staining,and PCR amplification and sequencing,and their evolutionary relationship was analyzed by phylogenetic tree.Additionally,dominant taxa at each classification level were counted and the diversity levels were analyzed.Results:Sixteen strains were isolated from Dermatophagoides farinae,including 14 Gram-positive bacteria and 2 Gram-negative bacteria.The cell morphology of 4 strains was short rod,1 strain was long rod and 11 strains were spherical.The 16 strains were identified as belonging to 3 phyla,3 classes,6 orders,7 families,9 genera and 16 species.The dominant phyla,class,order,family,genus and species were Actinomycetota(84.5%),Actinomycetes(84.5%),Micrococcus suborder(83.4%)and Micrococcaceae(83.1%),Kocuria(68.3%)and Kocuria massiliensis(68.3%),respectively.Conclusions:The culturable symbiotic bacteria of Dermatophagoides farinae have high diversity.Phylogenetic tree analysis indicates considerable evolutionary diversity among these bacteria.This study lays the foundation for subsequent genomic analysis of bacterial functional genes and the development of novel biocontrol strategies based on endosymbiotic bacteria.