番茄皮渣中酵母菌分离筛选及其发酵饲料工艺研究OA

The study aimed to screen yeasts with desirable properties,optimize the production process of tomato pomace fermented feed.Using tomato pomace as the research object,yeasts were isolated and purified from tomato pomace.The species of the isolated strains were identified by 28S rDNA gene sequencing,and their growth characteristics were investigated.Strains with good performance were selected to ferment tomato pomace,and the fermentation process was optimized.The results showed that a total of nine yeast strains were isolated in the experiment,including three strains of Saccharomyces cerevisiae,four strains of Pichia kudriavzevii,and two strains of Rhodotorula mucilaginosa.Among them,XM6,XM2,and XY3.1 exhibited good acid tolerance,while XM1,XM2,and XY3.2 showed good heat tolerance.The gas-producing capacity of Saccharomyces cerevisiae was superior to that of Pichia kudriavzevii,while Rhodotorula mucilaginosa showed the poorest gas-producing ability.The optimal fermentation conditions for tomato pomace were as follows:Moisture content of tomato pomace 85%,urea addition 1.66%,inoculum size 1×107 CFU/mL,the inoculation ratio of XY3.1 to XM2 of 3.55∶1.00,fermentation temperature 30℃,and fermentation time 72.5 hours.Under these conditions,the viable count in the fermented tomato pomace feed reached 4.53×108 CFU/g,and the crude protein content was extremely significantly increased by 38.27%compared with that before fermentation(P<0.01).The study shows that the yeasts isolated from tomato pomace possess good growth characteristics and stress tolerance,and after being fermented by it,the nutritional content of the tomato peel residue can be enhanced.