饲喂干白酒糟对关岭杂交牛肠道菌群结构和代谢谱的影响OA
Effects of dietary dried distiller's grains on intestinal flora structure and metabolic profile of Guanling crossbred cattle
试验旨在探究干白酒糟对关岭杂交牛肠道菌群及代谢谱的影响.选取12头健康的关岭杂交牛(关岭黄牛×西门塔尔牛),随机分为2组,每组6个重复,每个重复1头.对照组饲喂基础饲粮,替代组饲喂25%干白酒糟替代精料的饲粮.预试期15 d,正式试验期60 d.结果显示,与对照组相比,替代组中放线菌门相对丰度有所提高,拟杆菌门相对丰度有所降低,厚壁菌门和变形菌门相对丰度有所提高.替代组中hgcI进化枝、乳杆菌属和普雷沃氏菌NK3B31群相对丰度有所提高,拟杆菌属相对丰度有所降低.线性判别分析效应大小(LEfSe)分析显示,α变形菌纲及放线菌门菌属在替代组中得分最高,对照组则以拟杆菌门菌属为主.代谢组学共筛选出14种差异代谢物,其中鞘氨醇、牛磺胆酸、石胆酰牛磺酸、吲哚-3-乙醇等12种上调,L-酪氨酸和肉桂酸下调,这些代谢物富集于初级胆汁酸生物合成、胆汁分泌及牛磺酸代谢等16条通路.相关性分析表明,C39、瘤胃菌_UCG-014、hgcI进化枝等菌属与牛磺胆酸、鞘氨醇等代谢物呈正相关.研究表明,饲粮添加干白酒糟可以通过调节厚壁菌门、变形菌门、放线菌门及相关代谢物,参与纤维降解、脂代谢、肠道稳态及抗炎抗氧化过程,对关岭杂交牛生长育肥和肠道健康产生积极影响.
The experiment aimed to investigate the effects of dried distiller's grains on the intestinal flora and metabolic profile of Guanling crossbred cattle.Twelve healthy Guanling crossbred cattle(Guanling yellow cattle×Simmental cattle)were randomly divided into two groups,with six replicates per group and one cattle per replicate.The control group was fed a basal diet,while the replacement group was fed a diet in which 25%of the concentrate was replaced with dried distiller's grains.The pre-test period was 15 days,and the formal test period was 60 days.The results showed that compared with the control group,the replacement group exhibited an increased relative abundance of Actinobacteriota and a decreased relative abundance of Bacteroidota,while the relative abundances of Firmicutes and Proteobacteria were increased.In the replacement group,the relative abundance of the hgcI clade,Lactobacillus and Prevotellaceae NK3B31 group were increased,and the relative abundance of Bacteroides was decreased.Linear discriminant analysis effect size(LEfSe)analysis revealed that α Proteobacteria and Actinobacteria-relate d gener a ha d th e highes t LD A score s i n th e replacement group,whereas the control group was dominated by Bacteroidetes-related genera.Metabolomics analysis identified 14 differential metabolites,among which 12 metabolites,including sphingosine,taurocholic acid,lithocholyltaurine,and indole-3-ethanol,were significantly up-regulated,while L-tyrosine and cinnamic acid were down-regulated.These metabolites were enriched in 16 pathways,including primary bile acid biosynthesis,bile secretion,and taurine and hypotaurine metabolism.Correlation analysis showed that genera such as C39,Ruminococcus_UCG-014,and hgcI clade were positively correlated with metabolites including taurocholic acid and sphingosine.The study shows that dietary supplementation with dried distiller's grains may positively affect the growth,fattening,and intestinal health of Guanling crossbred cattle by regulating Firmicutes,Proteobacteria,Actinobacteria,and related metabolites,thereby participating in fiber degradation,lipid metabolism,intestinal homeostasis,and anti-inflammatory and antioxidant processes.
张青艳;张天天;朱二鹏;周碧君;杨颖;程振涛
贵州大学动物科学学院,贵州 贵阳 550025贵州大学动物科学学院,贵州 贵阳 550025贵州大学动物科学学院,贵州 贵阳 550025贵州大学动物科学学院,贵州 贵阳 550025贵州大学动物科学学院,贵州 贵阳 550025贵州大学动物科学学院,贵州 贵阳 550025
农业科技
干白酒糟关岭杂交牛肠道菌群结构差异代谢物
dried distiller's grainsGuanling crossbred cattleintestinal flora structuredifferential metabolites
《饲料研究》 2026 (8)
1-7,7
贵州省优秀青年科技人才项目(项目编号:黔科合平台人才[2021]5646号)贵州省科技计划项目(项目编号:黔科合基础-ZK[2022]一般158)
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