两种玛草蛉的线粒体基因组全序列分析及草蛉科系统发育OA

To clarify the complete mitochondrial genome(mitogenome)and systematic status of Mallada Navás,1925,the complete mitogenome of Mallada flavimaculus and Mallada viridianus were newly sequenced and annotated by Illumina second-generation sequencing technology.Furthermore,the characteristics of mitogenomes and the phylogenetic relationships within the family Chrysopidae were comparatively analyzed.The result showed that the complete mitogenome of M.flavimaculus and M.viridianus were 16 204 bp and 15 810 bp long,respectively.The AT content of the mitogenome of M.flavimaculus and M.viridianus were 80.14%and 79.16%.ATN was the mostly used start codon among the PCGs of mitogenome in Chrysopidae.Most PCGs used TAA as stop codons,while some PCGs terminated with TA-tRNA or T-tRNA.COX1 was the most conserved gene in the mitogenome of Chrysopidae,while ATP8 was a rapidly evolved gene.Almost all tRNAs could be folded into clover leaf structures,with the exception of tRNASer(AGN).Within Chrysopidae,Apochrysinae were recovered as the sister group to Chrysopinae+Nothochrysinae.Within Chrysopinae,Nothancylini were recovered as the sister group to(Leucochrysini+Belonopterygini)+(Ankylopterygini+(Nineta+Chrysopini)).Mallada were recovered as the sister group to Chrysoperla.These findings not only contribute foundational data for molecular systematic studies in Chrysopidae but also aid in elucidating the differentiation patterns of Chrysopidae mitogenomes.