基于转录组学探讨天麻乙酸乙酯提取物治疗肌少症的作用机制OA

Objective Based on transcriptomics,Caenorhabditis elegans was used to replicate a dexamethasone-induced sarcopenia model to explore the preventive and therapeutic effects of EtOAc extract from Gastrodia elata(EEGE)on sarcopenia.Methods L4-stage N2 worms were placed on corresponding experimental plates to complete drug administration and model replication.Muscle function was detected by swimming and crawling tests,and muscle state was observed by phalloidin staining.Reactive oxygen species(ROS)fluorescence staining,nitric oxide(NO)content,antioxidant enzyme activity,and total antioxidant capacity were measured to reflect the level of oxidative stress.Transcriptomics and PCR-based microarrays were used to screen for possible mechanisms of EEGE in treating sarcopenia.Results Compared with the model group,EEGE treatment significantly increased swimming and crawling frequencies(P<0.01,P<0.001).After EEGE treatment,the body wall muscle stripes of the worms were clear and regularly arranged.EEGE effectively combated oxidative stress damage,significantly reducing ROS and NO levels in the worms(P<0.001)while increasing antioxidant enzyme activity and total antioxidant capacity(P<0.05,P<0.001).Transcriptomics analysis suggested that EEGE may alleviate sarcopenia by promoting autophagy,upregulating glutathione metabolism-related pathways,and regulating biological processes such as the cell matrix and cuticle.Conclusion The results indicate that EEGE significantly improves muscle function and state in worms,and has potential therapeutic effects on sarcopenia.