肿瘤三维细胞球与类器官代谢物质谱成像研究进展OA

Three-dimensional tumor spheroids and organoids can effectively simulate the spatial heterogeneity and microenvironmental characteristics of tumors in vivo,and have emerged as important models in tumor metabolism research.Mass spectrometry imaging(MSI)technology enables in situ analysis of the distribution of metabolites,lipids,and drugs while preserving the spatial information of tissues.The integration of three-dimensional tumor models and mass spectrometry imaging allows for the precise analysis of tumor metabolic reprogramming and its spatial heterogeneity.This review systematically summarizes the methods for relative model construction and the sample preparation strategies tailored for MSI.It also introduces the core techniques features of MSI,with a particular focus on its applications progress in combination with three-dimensional tumor models for elucidating spatial metabolic heterogeneity characteristics of tumors,molecular mechanism,and the spatial distribution of drugs.This work aims to provide methodological guidance and theoretical support for studies on tumor metabolic mechanisms and drug development.