上海市孕妇膳食赭曲霉素A暴露评估OA

[Objective]To investigate the contamination status of ochratoxin A(OTA)in commercially available food products in Shanghai,and to assess OTA exposure levels and the associated non-carcinogenic and carcinogenic risks among pregnant women by integrating dietary consumption data of this population.[Methods]The levels of OTA contamination in 1 520 food samples collected in Shanghai from 2022 to 2023 were determined using liquid chromatography-tandem mass spectrometry.An exposure assessment model was developed based on the dietary consumption levels of pregnant women from the 2016-2017 Shanghai Pregnant Women Dietary Monitoring Survey to calculate the estimated daily intake(EDI)of OTA,the margin of exposure for non-carcinogenic toxicity(MOE1),and the margin of exposure for carcinogenic toxicity(MOE2).An MOE1 greater than 200 and an MOE2 greater than 10 000 indicate that the non-carcinogenic toxicity and carcinogenic toxicity resulting from exposure are negligible,respectively.For samples with OTA contamination levels below the limit of detection(LOD),which accounted for more than 80％of the samples,the OTA levels were assigned values of 0 and LOD,respectively,for subsequent calculations.[Results]The detection rates of OTA in cereals,nuts,dried fruits,and alcohol samples collected in 2022 were 2.03％,0,0,and 0,respectively.The OTA detection rates in cereals,nuts,dried fruits,beans,and alcohol samples collected in 2023 were 2.50％,0.39％,2.47％,1.67％,and 13.33％,respectively.For pregnant women in Shanghai in 2022,simulation results indicated that when assigning a value of 0 and the LOD,the P50 values of EDI for dietary OTA exposure were 0.05 and 0.72 ng·(kg·d)-1,respectively,and the P95 values of EDI for dietary OTA exposure were 0.25 and 2.40 ng·(kg·d)-1,respectively.For pregnant women in Shanghai in 2023,the P50 values of EDI for dietary OTA exposure were 0.04 and 1.00 ng·(kg·d)-1,respectively,and the P95 values of EDI for dietary OTA exposure were 0.23 and 2.67 ng·(kg·d)-1,respectively,both substantially below the tolerable daily intake(TDI)for OTA[17 ng·(kg·d)-1].The EDI for dietary OTA exposure in 100.0％of Shanghai pregnant women was lower than the TDI,indicating an overall low level of dietary OTA exposure among this population.For 100.0％of pregnant women,the MOE₁ for dietary OTA exposure exceeded 200.When assigned a value of 0,the MOE₂ for 100.0％of pregnant women in both 2022 and 2023 exceeded 10 000.When assigned the LOD value,72.3％and 81.8％of pregnant women in 2022 and 2023,respectively,had an MOE₂ exceeding 10 000.[Conclusion]From 2022 to 2023,samples of cereals,nuts,dried fruits,beans,and alcohol sold in Shanghai exhibited varying degrees of OTA contamination.The overall EDI of OTA exposure among pregnant women in Shanghai remained at a low level.The non-carcinogenic and carcinogenic risks associated with OTA exposure were generally low and at controllable levels.