基于液相色谱-质谱的西洋参不同部位人参皂苷差异性分析OA

Ginsenosides are important chemical components of Panax quinquefolius L.and are closely related to its pharmacological activities.In this study,ultra-high performance liquid chromatography-mass spectrometry(UHPLC-MS)was used for high-throughput analysis of ginsenosides in the roots,leaves,and seeds of Panax quinquefolius L.,leading to the identification of 72 ginsenosides across these parts.Both univariate and multivariate analyses revealed significant differences in the content and types of ginsenosides among the different plant parts.Based on statistical significance(p＜0.05)and biological significance(fold change＞4),23 differential saponins were identified in the roots vs.leaves comparison,26 in the roots vs.seeds comparison,and 27 in the leaves vs.seeds comparison.Content analysis revealed that the total amounts of Rg1,Re,and Rb1 in the leaves and roots were roughly equivalent and higher than in the seeds,while most other ginsenosides were more abundant in the leaves and roots than in the seeds.The pseudo-ginsenoside F11,which is unique to Panax quinquefolius L.,was most abundant in the leaves,whereas acetylated pseudo-ginsenoside F11 was most abundant in the roots.20(S)-Ginsenoside Rh1,acetylated ginsenoside Rg1,and quinquenoside Ⅳ were all significantly different among the three parts and could serve as markers for differentiation.This study reveals distinct ginsenoside profiles in the roots,leaves,and seeds of Panax quinquefolius L.,providing technical support for its efficient development and utilization.