CEACAM19调节PI3K/Akt通路改善胃癌免疫微环境的研究OA

Objective To investigate the role of CEACAM19 regulating the PI3K/Akt pathway in improving the immune microenvironment of gastric cancer.Methods The CEACAM19 was detected in gastric cancer tissues and paired adjacent tissues using RT-qPCR,and its correlation with clinical pathological parameters was analyzed.HGC-27 cells were treated with CE AC AM1 9 siRNA or empty lentiviral vector to establish tumor-bearing mouse models,which were randomly divided into control group,CEACAM19 knockdown group,empty vector group,and CEACAM19 knockdown+740 Y-P group.Tumor volume and mass were measured after intervention with PI3K activator 740 Y-P.CEACAM19 and PI3K/Akt were detected by RT-qPCR and immunoblot-ting.Peripheral blood immune cell differentiation was assessed by flow cytometry.Serum immune cytokine were measured by ELISA.NK cell and macrophage cytotoxicity in the immune microenvironment were evaluated using the CCK-8 assay.Results CEACAM19 were associated with lymph node metastasis,invasion depth,and clinical stage in gastric cancer patients.Compared with the control group,the CEACAM19 knockdown group showed increased proportions of CD4+and CD8+T cells,M1-type mac-rophages,IL-6 and IL-2 levels,and NK cell and macrophage cytotoxicity(P＜0.05),while decreased proportions of M2-type mac-rophages,CEACAM19,p-PI3K/PI3K and p-Akt/Akt,tumor volume and mass,and TGF-β1 and IL-10(P＜0.05).740 Y-P atten-uated the effects of CEACAM19 knockdown on the aforementioned indicators in gastric cancer mice.Conclusion CEACAM19 knockdown could inhibit the activation of the PI3K/Akt signaling pathway,thereby modulating the immune microenvironment of gastric cancer and enhancing the cytotoxicity of immune cells against tumor cells,making it a potential novel biomarker for gastric cancer.