槟榔种质资源农艺及品质性状InDel标记关联分析OA

Arecanut(Areca catechu L.)is an important tropical specialty economic crop in Hainan Province.It is listed as the first of the four south China medicinal plants and holds a significant position in Hainan's agricultural production.Uncovering molecular markers associated with arecanut agronomic and quality traits has great significance for breeding new high-quality varieties with desirable traits,and molecular marker-assisted breeding.In the research,50 pairs of InDel markers were utilized to genotyping 149 arecanut germplasm resources,and the genetic diversity and population structure analysis were also conducted.The association analysis was performed using both the GLM and MLM associa-tion analysis models to identify the molecular markers associated with phenotypic traits.The results showed a total of 123 allele loci were detected in the population.The average observed heterozygosity(Ho)and expected heterozygosity(He)was 0.36 and 0.43 respectively.The average Shannon's information index(I)was 1.1548,and the polymorphism information content(PIC)of the primers was ranged from 0.15 to 0.52,with an average of 0.35.88.00%of the primers exhibited moderate to high polymorphism,which indicating that the InDel primers in this study had high polymorphism.The population structure analysis suggested that 149 arecanut divided into three subgroups,which consisting of 118,24 and 7 materials respectively.Using the GLM and MLM association analysis models,58 and 40 associated loci were detected,with phenotypic variation explanation rates ranging from 4.36%to 13.39%and 4.35%to 9.29%respectively.All associated loci detected by the MLM model were also detected by the GLM model.The markers AC1505 and AC1519 were detected to be associated with fruit shape and quality-related traits in both methods,exhibiting a signifi-cant"pleiotropic effects"phenomenon.The findings would provide a theoretical basis for gene mining related to are-canut traits and breeding for quality characteristics.