辣椒NAM基因家族的全基因组鉴定及其在果实成熟期的表达分析OA

The NAM gene family play an important regulatory role in plant growth and development,organogenesis and abiotic stress responses.In order to study the regulatory function of NAM gene family members during fruit develop-ment,bioinformatics was used to identify the NAM gene family of pepper,and its protein physicochemical properties,phylogenetic relationships,chromosomal localization,conserved motif distribution,gene structure,species collinearity and gene expression were analyzed in detail.The results showed that there were 31 NAM gene family members distri-buted on 11 chromosomes,and there was a phenomenon of uneven distribution.Through phylogenetic analysis,the NAM gene family members of pepper were divided into five subfamilies.Gene structure analysis showed that most CaNAM genes contained 5 exons.Collinearity analysis showed that the gene collinearity between pepper and tomato was closer than that between pepper and Arabidopsis,and there were two additional collinearity segments between pepper and tomato,indicating that their evolutionary relationship was closer.The gene expression profile showed that the expression of the six CaNAM genes showed high expression levels in the four stages of the color transition process of pepper fruits,especially the expression of CaNAM3 gene was the highest when the color change reached 50%,which was consistent with the transcriptome data.Notably,the CaNAM28 gene showed a specific and significant upregulation during the red ripening stage,indicating that it may be a potential candidate gene involved in the regulation of fruit ri-pening.Through the preliminary identification and functional analysis of NAM gene family members in pepper,it would provide a research basis for further exploring the regulatory function of NAM gene family members in the de-velopment and fruit color transition of pepper.