蔗茅EfLRK10L-2-8基因的克隆及表达模式分析OA

Members of the RLK gene family are widely involved in responses to adversities such as drought and low temperature.Erianthus fulvus is a wild sugarcane species with excellent stress resistance traits.Cloning and analyzing LRK10L family member genes from E.fulvus holds significant reference value for mining stress resistance genes in sugarcane and for sugarcane stress resistance breeding.In this study,an LRK10L-type receptor-like kinase gene,EfLRK10L-2-8,was cloned from E.fulvus.Sequence analysis revealed it contained a complete coding sequence(CDS)of 1998 bp,encoding 665 amino acids.The predicted molecular weight of the protein was approximately 74.91 kDa,with an isoelectric point of 6.03.Domain analysis indicated that the protein contained a cysteine-rich extracellular do-main,a transmembrane domain,and an intracellular serine/threonine kinase domain,consistent with the typical struc-tural features of LRK10L-type receptor-like kinases(LRK10L-RLKs).Phylogenetic analysis showed that EfLRK10L-2-8 was most closely related to ZmL RK10L-1 from maize and OsLRK10L-3 from rice,suggesting it may have conserved functions in graminaceous crops.Subcellular localization results demonstrated that the EfLRK10L-2-8 protein was pri-marily localized to the cell membrane in tobacco leaf cells.qRT-PCR analysis showed that this gene was highly ex-pressed in the roots,stems,and leaves of E.fulvus;its expression level was significantly induced and upregulated under drought and low-temperature stress treatments,suggesting it may positively participate in related stress signaling re-sponses.The results indicate that EfLRK10L-2-8 plays an important role in the response of E.fulvus to drought and low-temperature stress,providing an important candidate gene resource for elucidating the stress resistance molecular mechanisms of E.fulvus and for sugarcane stress resistance breeding.