菠萝4CL基因家族的鉴定及表达分析OA

4-coumaric acid:CoA ligase(4CL)gene regulates the biosynthesis of lignin and flavonoids,playing an im-portant role in plant organ color formation,disease resistance,and stress resistance.In order to understand the molecular characteristics and biological functions of the pineapple 4CL gene family,this study used bioinformatics technologies and tools to identify and analyze members of the 4CL gene family in the pineapple genome,followed by experimental validation.A total of 30 4CL genes were identified from the pineapple genome,and the physicochemical properties such as amino acid number,molecular weight,and isoelectric point were variant.Members of the Ac4CL gene family were unevenly distributed on 13 out of 25 chromosomes in pineapple.There were 5 pairs of genes exhibiting intraspecific collinearity,and 6 pairs of genes exhibiting collinearity between pineapple and Arabidopsis.There were significant dif-ferences in the expression of Ac4CL in pineapple at 60,90 and 120 days after flowering.Among them,Ac4CL2 showed increasingly significant differences in expression after flowering.Furthermore,the gene also showed significant differ-ences in expression among four different pineapple varieties,namely Jinzuan,Milk,Watermelon,and Bali,and it is inferred that they are closely related to the accumulation of anthocyanins during fruit ripening.This study would provide a theoretical basis for further exploring the function of the pineapple 4CL gene family.