双孢蘑菇菌株HK和A15转录组差异分析OA

To obtain the gene expression differences between Agaricus bisporus strains A15 and HK at the mycelial stage and fruiting body stages,and analyze the molecular mechanisms causing their phenotypic differences,transcriptome analysis was used to compare the gene expression differences between Agaricus bisporus strains A15 and HK at the mycelial stage and fruiting body stage,and KEGG(Kyoto Encyclopedia of Genes and Genomes)enrichment analysis was performed on the dif-ferentially expressed genes.The results showed that the growth rate of A15 at the mycelial stage was significantly lower than that of HK,and the differential genes were significantly enriched in pathways such as oxidative phosphorylation and steroid biosynthesis.Oxidative phosphorylation affected the mycelial growth rate by regulating the efficiency of ATP supply.At the fruiting body stage,the pileus of A15 was more prone to scaling,and the differential genes were mainly enriched in pathways such as galactose metabolism and glycerol metabolism.It was speculated that these metabolic pathways affected the pileus morphology by regulating the synthesis and remodeling of cell wall polysaccharides.This study reveals the positive regulatory effect of oxidative phosphorylation on mycelial growth,as well as the key role of galactose and glyceride metabolism in fruit-ing body morphogenesis,providing a theoretical basis for the directed breeding of Agaricus bisporus.