基于16S rRNA的奶牛乳汁及环境中菌群结构分析OA
Analysis of microbial community structure in cow milk and environment based on 16S rRNA
为探究奶牛乳房炎致病菌对乳汁菌群的影响及来源,采用 16S rRNA测序技术,采集山东省烟台市某奶牛场患乳房炎、健康奶牛乳汁及挤奶厅、牛舍空气和粪便样本,对比菌群结构并通过 SourceTracker溯源.研究表明,乳房炎患病奶牛乳汁中细菌数量增加,种类减少.同一来源的样品样本组成基本相同,健康奶牛、患乳房炎奶牛和粪便中样本组成差异显著.同时,结果表明乳房链球菌(Streptococcus uberis)是该试验牛场奶牛乳房炎主要致病菌,奶牛患乳房炎后菌群功能发生变化.通过 SourceTracker 软件溯源,推测致病菌主要来源于挤奶厅环境.结果为奶牛乳房炎防治提供了一定的理论参考.
To investigate effects and source of pathogens causing bovine mastitis on microbial community in milk,16S rRNA sequen-cing technology was employed.Samples were collected from cows with mastitis and healthy cows at a dairy farm in Yantai City,Shan-dong Province,as well as from milking parlour,cowshed air,and feces.Microbial community structures were compared,and sources were traced using SourceTracker.Results showed that milk of cows with mastitis increased bacterial abundance but reduced diversity.Samples from the same source had similar compositions,while significant differences were observed among samples from healthy cows,mastitis-affected cows and faeces.Moreover,results indicated that Streptococcus uberis was the main pathogen causing mastitis at stud-ied farm,with changes in milk microbiota following mastitis.Source tracking with SourceTracker suggested that pathogens mainly ori-ginated from milking parlor environment.These results provide a theoretical reference for prevention and control of mastitis in dairy cows.
王凯;宋宗润;王光暄;王淑祺;杨政超;张浩洋;鲍鹏天;南松剑
中国农业大学烟台研究院,山东 烟台 264670中国农业大学烟台研究院,山东 烟台 264670中国农业大学烟台研究院,山东 烟台 264670中国农业大学烟台研究院,山东 烟台 264670中国农业大学烟台研究院,山东 烟台 264670中国农业大学烟台研究院,山东 烟台 264670中国农业大学烟台研究院,山东 烟台 264670中国农业大学烟台研究院,山东 烟台 264670
农业科技
奶牛乳房炎乳房链球菌菌群结构病原菌溯源16S rRNA
bovine mastitisStreptococcus uberismicrobial community structurepathogen traceability16S rRNA
《农业工程》 2026 (5)
64-71,8
2023年烟台市校地融合发展项目(2023XDRHXMPT12)
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